Molecular characterization and phylogenetic analyses of Tripartiella macrosoma Basson and Van As, 1987 and Tripartiella obtusa Ergens and Lom, 1970 primarily based on 18S rRNA gene sequence information
Within the current research, we supplied the primary 18S rRNA gene sequence information of two Tripartiella species, Tripartiella macrosoma Basson and Van As, 1987 and Tripartiella obtusa Ergens and Lom, 1970, which had been remoted from Tachysurus fulvidraco (Richardson, 1846) and Hemibarbus maculatus Bleeker, 1871 in Chongqing, China, respectively. Morphologically, each species fall throughout the morphometry vary of the unique descriptions and are similar to the unique populations within the general look of the adhesive disc. Tripartiella macrosoma could be simply distinguished from the opposite Tripartiella species by possessing the denticle with a protracted strip and conspicuously inclined backward blade and a sturdy and quick ray.
Tripartiella obtusa is especially characterised by a broad blade and a comparatively lengthy ray. Phylogenetically, T. macrosoma clustered with Trichodinella myakkae (Mueller, 1937) Raabe, 1950 and additional with Trichodinella sp., which was sister to a bunch that features 4 populations of Trichodinella epizootica (Raabe, 1950) Šrámek-Hušek, 1953; lastly, they shaped a small clade with T. obtusa. This consequence urged that T. macrosoma had a more in-depth relationship with Trichodinella spp. than with T. obtusa and T. obtusa diverged sooner than T. macrosoma and Trichodinella spp. By combining morphological and molecular information, the polyphyletic traits of Tripartiella and Trichodinella had been additional analyzed, and the outcomes revealed that the validity of the genus Tripartiella is uncertain.
MOLECULAR INVESTIGATION AND PHYLOGENETIC ANALYSIS OF ANAPLASMOSIS IN DOGS
Anaplasmosis is a widespread vector-borne illness affecting canines, and Anaplasma platys is the foremost etiological agent of the illness. The research examines anaplasmosis molecular prevalence, associated danger components, and alteration of hematological variables in Anaplasma-affected canines. A complete of 150 blood samples had been collected from canines within the district of Lahore, Pakistan. The samples had been screened with PCR concentrating on the 16S rRNA gene of Anaplasma. Sequencing of samples that had been discovered optimistic after performing PCR was carried out. A questionnaire was developed to gather epidemiological information on topic canines, and the data was analyzed with a logistic regression mannequin utilizing SPSS.
The present research revealed an 11.34% (17/150) prevalence of anaplasmosis in canines primarily based on PCR detection. Tick infestation, earlier tick historical past, home hygiene, and tick management standing had been main danger components linked with illness prevalence. Pink blood cell rely, packed cell quantity, hemoglobin, and platelet rely had been decreased considerably (P < 0.05) in Anaplasma-infected canines. Phylogenetically, the two isolates of the present research clustered collectively, and that cluster was similar to A. platys isolates from India, Malaysia, and Thailand.
New association of three genera of fish tapeworms (Cestoda: Proteocephalidae) in catfishes (Siluriformes) from the Neotropical Area: taxonomic implications of molecular phylogenetic analyses
Tapeworms (Cestoda: Proteocephalidae) are the dominant element of communities of intestinal parasites in pimelodid and different catfishes (Siluriformes) from South America. Although these parasites have been studied intensively over multiple century, molecular taxonomy and phylogenetics have questioned their morphology-based classification, thus elevating doubts concerning the systematic worth of traits generally used to circumscribe particular person taxa. Within the current research, members of three morphologically well-characterized genera of proteocephalids from pimelodid (Hemisorubim platyrhynchos and Sorubim lima) and auchenipterid (Ageneiosus inermis) catfishes from the Paraná or Amazon River basins had been subjected to DNA sequencing of the big subunit nuclear ribosomal RNA (lsrDNA) and full mitochondrial cytochrome c oxidase subunit I (COI).
Phylogenetic analyses revealed the sister relationship between Manaosia bracodemoca and Mariauxiella piscatorum, and amongst Mariauxiella pimelodi and Ageneiella brevifilis. In consequence, Mar. piscatorum and A. brevifilis are transferred to Manaosia and Mariauxiella, respectively, as Manaosia piscatorum n. comb. and Mariauxiella brevifilis n. comb., and the genus Ageneiella is suppressed. Diagnoses of Manaosia and Mariauxiella are amended. In addition, the current research revealed misidentification of tapeworms whose sequences are deposited within the GenBank database.
Canine Visceral Leishmaniasis in São Paulo, Brazil, the Most Populous Metropolis of South America: Isolation, Molecular Prognosis, and Phylogenetic Inferences
Background and Targets: Canine visceral leishmaniasis impacts canines, the principle city reservoirs, which favor the transmission and growth of this zoonotic illness in areas with excessive anthropization course of and human density. We investigated the occurence of Leishmania infatum primarily based in molecular prognosis, and phylogenetic evaluation of isolates obtained from canines in metropolitan area of São Paulo. Strategies: A complete of 201 canines had been examined by parasitological and molecular prognosis. Phylogenetic evaluation primarily based sequences from SSUrDNA and gGAPDH genes had been carried out. Outcomes: The parasitological prognosis revealed 5% (10/201) of positivity, and the sequences obtained from seven isolates had been clustered with L. infantum in phylogentic evaluation primarily based on SSUrDNA and gGAPDH genes. A complete of 24.9% (50/201) of canines had been optimistic in molecular prognosis primarily based on cathepsin L-like marker. Interpretation and Conclusion: In keeping with this research, it’s essential to implement a surveillance coverage of visceral leishmaniasis, intensifying the actions of prognosis, prevention, and management of this zoonosis.
Morphological Description, Phylogenetic and Molecular Evaluation of Dirofilaria immitis Remoted from Canines within the Northwest of Iran
Background: Dirofilariasis is a globally distributed arthropod-borne parasitic illness of primarily canids and felids. We evaluated to increase the information of morpho-molecular traits and outer ultrastructure of Dirofilaria immitis remoted from Northwest of Iran.
Strategies: Total, 67 filarial worms together with 41 females and 26 males parasites had been collected from the cardiovascular system of the 43 stray canines in Meshkinshar, Ardebil Province, Northwest of Iran in 2017, and subjected to mild and scanning electron microscopy (SEM) in addition to carmine alum staining for morpho-molecular and identification. Molecular strategies had been used for affirmation of morphological findings by sequencing of Cyto-chrome c oxidase subunit I (cox1) gene.
Outcomes: The partial DNA sequencing of cox1 gene of grownup parasites confirmed appreciable homology and shut proximity to the beforehand remoted from Kerman and Meshkinshahr, Iran. The bottom genetic variation and the best intra-species variability was present in D. immitis and Dirofilaria repens, respectively. No similarity was recognized between D. immitis nucleotide sequence and Wolbachia species as its endosymbiont micro organism.
Conclusion: The SEM method is a wonderful device for differential recognition of the parasite floor morphology and molecular strategies may differentiate and determine Dirofilaria spp.